产品货号：
GS2148
中文名称：
Mly113I内切酶
英文名称：
Mly113I
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity.
技术规格
相关搜索：Mly113I内切酶，Mly113I限制性内切酶
使用说明
High enzyme concentration may result in star activity.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof T7 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GG↑CGCC |
| 来源 | Micrococcus lylae 113 |
| Assayed on | T7 DNA |
| Optimal buffer | B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃. |
| Ligations | After 3-fold overdigestion with enzyme more than 80%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof T7 DNA with 10 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BbeI?^,DinI^,EgeI^,KasI^,NarI,PluTI^,SfoI^,SspDI^ |
相关搜索：Mly113I内切酶，Mly113I限制性内切酶